"The Sulphate Reducing Bacteria" by John Postgate has recipes for several
media.
>From ASM's "Manual of Methods for General Bacteriology (1981)
yeast extract 1.0g
sodium lactate 4.0g
NH4Cl 0.5g
K2HPO4 1.0g
MgSO4 . 7H2O 0.2g
CaCl2 . 2H2O 0.1g
FeSO4 . 7H2O 0.1g
Na2SO4 0.5 g
(NaCl, for marine organisms 20-30g)
Oddly they don't give a volume but per litre would seem to be appropriate.
--
Austin Reade
tegs <tegs at home.nl> wrote in message
news:3G%f6.95137$Wa6.2075171 at zwoll1.home.nl...
> hello,
>> i hope someone can help me,
> i need the defined culture medium for
> sulfate reducing bacteria for a project in the
> first year of my biology study. i looked on the net
> for a few hours but i found nothing but commercial
> biotechnological sites. i thought that someone here
> could help me out? I need to know the chemical
> substances to form the medium and the quantity..
>> thanks i really appreciate it!
>>>
