What is Clontech's "TTC"?

Raymond Cox r.p.cox at bmb.sdu.dk
Thu Feb 1 18:13:51 EST 2001

In message <Pine.GSO.3.96.1010201173806.27885D-100000 at mbcrr>
          bsturner at mbcrr.harvard.edu (Bradley Turner) wrote:

> Hello Bionetters,
> Does anyone know the identity of the "TTC"
> reagent in Clontech's new CLONdiscs.
> Evidently when used in screening 
> transformed Ecoli lac+ will appear blue
> and lac- appear red.  It seems more definitive
> that blue/white.  I couldn't find any thing
> on Medline with TTC.
TTC usually refers to triphenyltetrazolium chloride. It is
reduced during bacterial metabolism to an insoluble red formazan.

Raymond Cox
Department of Biochemistry and Molecular Biology
Odense University Campus - University of Southern Denmark

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