In message <Pine.GSO.3.96.1010201173806.27885D-100000 at mbcrr>
bsturner at mbcrr.harvard.edu (Bradley Turner) wrote:
>> Hello Bionetters,
>> Does anyone know the identity of the "TTC"
> reagent in Clontech's new CLONdiscs.
> Evidently when used in screening
> transformed Ecoli lac+ will appear blue
> and lac- appear red. It seems more definitive
> that blue/white. I couldn't find any thing
> on Medline with TTC.
>TTC usually refers to triphenyltetrazolium chloride. It is
reduced during bacterial metabolism to an insoluble red formazan.
Raymond Cox
Department of Biochemistry and Molecular Biology
Odense University Campus - University of Southern Denmark
