I read a paper where the researchers suggest that the insertion of
transposons are random and that the extensive recovery of cells with
insertion of transposons in the intergenic regions is due to the fact that
many of these regions are non-essential.
1) I am trying to think of an experiment that would support the hypothesis
that no transposon insertions were identified in an essential gene (ie a
gene involved in protein translocaion) because cells harboring these
insertions are not viable, rather than that the transposon has preferred
regions it inserts into...Have any ideas?
2) How about a follow-up experiment that could be preformed to identify
virulence factors?
Thanks
