"Halogen" <halo &anti spam& gen at tpg.com.au> wrote in message
news:3b761bcb at dnews.tpgi.com.au...
> Basically I have an undergraduate project to test this new idea for a
>> Does anyone know a simple way of quantitatively measuring a product
> by bacteria without resorting to using equipment like HPLC?
>> I've tried measuring H2S produced by sulphate reducing bacteria, but this
> difficult to work out since it's only practical to measure it as a gas and
> lot is staying dissolved in the liquid in the bioreactor.
Then why not measure the dissoved sulphide? There's colour methods and dip
sticks available (see your Merck catalogue for a start).
> I've tried using pigment produced by psuedomonas fluorescens, but this
> didn't work due to this bacteria only producing pigment under certain
>> Any other ideas? I'm really looking for a bacteria I can easy assay a
> metabolic product of using a colour reaction and a spectrophotometer, but
> can't seem to find a way to do this anywhere.
>Nitrite production by ammonia oxidizers is easy to follow, as is ammonia
production by dissimilatory nitrate reducers.