Has anyone tried to fetch a phage from a
commercial lysogen like Novagen BL21(DE3)
E coli (T7 RNApol)?
For that matter, has anyone tried to fetch
the pLysS (plasmid??) giving the lysozyme
and chloramphenicol resistance from the
BL21(DE3)pLysS strain?
I had occasion to do the above recently
and the latter (pLysS), at least, was a
failure, just growing and doing a miniprep.
No plasmid could be found at all.
I could see that maybe the lysogen will not
ever make a complete new phage again, but I
am not sure what happened to the vector for
lysogen and chloramphenicol that it can't
be fetched from a miniprep.
Any ideas?
Cheers,
Dominic
North 59 37' 30"
East 17 48' 10"
