Bacitracin and Group A Streps

John Gentile yjgent at home.com
Wed Aug 1 17:55:32 EST 2001

This lab has used horse blood since before I came to work here. I don't know
the particulars, but we see a fair number of Haemophilus in our population.
(btw, I started here in Jan, 1981)
John Gentile                                    Rhode Island Apple Group
yjgent at home.com                                      President
 "I never make mistakes, I only have unexpected learning opportunities"

> From: Dilworth <bactitech at hortonsbay.com>
> Organization: Infinite Systems - Columbus, OH
> Newsgroups: bionet.microbiology
> Date: Wed, 01 Aug 2001 00:46:30 -0400
> Subject: Re: Bacitracin and Group A Streps
> Why did you make the switch?  I've only ever used TSA with 5% sheep
> blood for primary media, although I remember reading somewhere that
> horse blood is the standard in Australia (why I don't know, as they have
> LOTS of sheep in Australia - maybe someone from Au can answer
> that....).  I would suspect that the Columbia base also enables them to
> grow somewhat larger than they do on sheep blood plates.
> Thanks for the information. I had no idea they would look differently.
> Judy Dilworth, M.T. (ASCP)
> Microbiology
> John Gentile wrote:
>> Judy,
>> Actually we see quite a few of them since we use Columbia with 5% Horse
>> Blood Agar as our primary blood plate. Many Enterococcus feacalis are Gamma
>> or Alpha hemolytic on Sheep Blood, but show a nice Beta hemolysis on Horse.
>> Horse is also good for growing Haemophilus without additional growth
>> factors.

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