Dcm- Rec A- strain ?

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Tue Apr 10 08:28:10 EST 2001

In article <3AD2D8E8.F5EAEE89 at cmm.ki.se>, the eminent Eric DUFOUR at
>Does anyone know about such a strain ?

All E.coli B strains are dcm-.

Have you looked at Invitrogen or Stratagene for recA and maybe hsdR
derivatives of JM110. I suspect you will have problems on both. You will
find endA derivatives.

BL21 is a B derivative that is dcm- and r-m- but not recA.

So if they are no use then go to the E.coli Stock Genetic Centre and
search that online for what you want. 

Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK 


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