In article <3AD2D8E8.F5EAEE89 at cmm.ki.se>, the eminent Eric DUFOUR at
wrote
>Does anyone know about such a strain ?
All E.coli B strains are dcm-.
Have you looked at Invitrogen or Stratagene for recA and maybe hsdR
derivatives of JM110. I suspect you will have problems on both. You will
find endA derivatives.
BL21 is a B derivative that is dcm- and r-m- but not recA.
So if they are no use then go to the E.coli Stock Genetic Centre and
search that online for what you want.
Duncan
--
Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR:
Optimisation, Probe Technology & Future Systems
4-5 September 2001
King Alfred's College, Winchester, UK
http://www.dera.gov.uk/html/news/events/real_time_quantitative_pcr.htm
