Hi,
I have a question concerning different plaque morphology coming from
lysogenic bacteriophages.
In a standard plaque assay for the quantification of a lambda phage lysate,
the plaques formed after an overnight incubation appear as circles with a
clear center and a turbid periphery where grow of lysogenic bacteria is
clearly visible. In turn, when the plaque assay is performed with P22 phage,
plaques appear with a turbid center (growth of lysogens) and a clear
periphery. Does anibody know the reason of these differences in plaque
appearance?
Thanks in advance!
Carles Borrego
Microbiology Section, Institute of Aquatic Ecology
University of Girona
Campus de Montilivi, E-17071, Girona (Spain)
