Justin Doward wrote:
> Try looking up the polymerase chain reaction (pcr) for primer extension
> information, and I think chromosomal walk technique is referring to the way
> in which enzymes (such as dna polymerase etc) move along dna so look up DNA
> replication.
>
!!??? Where did you find that information???
Primer extension is a technique to find the transcription iniation nucleotide.
Its based on the amplification of the messenger RNA using the retrotranscriptase
(not a PCR enzyme, since none of them are termostable), and comparing the size
of the amplified cDNA with a sequence of the gene (both performed using the same
primer).
Chromosome Walking is a technique to clone adjacent fragments of DNA from a
chromosome. There are different methods, based on labelled probes or insertion
of marker genes.
Try any molecular techniques book...
Sergio
