You should never use an inhibitory agar to start to revive a freeze dried
organism. If you received it from a company check the package insert for the
directions and the recommended media. I would always take frozen organisms
and put them on a plain blood agar plate and then subculture to BAP for the
next 3 days. Then you can try the pseudo type agars. Most pseudos like to
grow at a little less than body temp - try a 30º C incubator, although a
35ºC should work too. Pseudos are aerobic so they should be in ambient air
instead of CO2.
--
John Gentile Rhode Island Apple Group
yjgent at home.com Past President, Publicity Chairman
"I never make mistakes, I only have unexpected learning opportunities"
> From: ramdzani at fsas.upm.edu.my (Ramdzani Abdullah)
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Newsgroups: bionet.microbiology
> Date: 29 Nov 2000 06:58:40 -0000
> Subject: pseudomonas fluorescens P17
>> Can anyone tell me if Cetrimide agar suitable for the growth of Ps P17?
> and what is the optimum temperature and suitable medium to revive
> freeze dried pseudomonas fluorescens P17 ?
> thanks!
> jenni
>>> ---
