We've always set up 0.5 mls on a blood agar plate and incubated for 48 hrs.
We used a colony count of <200 for H20 and <2000 for dial fluid. We would
only identify organisms if there was a single colony type greater than the
acceptable limits. Even if the cc was high but still acceptable the dialysis
people would bleach out the machines - or what ever they needed to do. A
repeat culture a day or 2 later would almost always be CC <2/ml.
--
John Gentile Rhode Island Apple Group
yjgent at home.com Past President, Publicity Chairman
"I never make mistakes, I only have unexpected learning opportunities"
> From: "Simon Lee" <pedlcy at orgio.net>
> Organization: Kosin Medical Center
> Reply-To: "Simon Lee" <pedlcy at orgio.net>
> Newsgroups: bionet.microbiology
> Date: Wed, 15 Nov 2000 10:30:01 +0900
> Subject: Help ! the media for Q.A of dialysis water or distilled water.
>> Is ther anyone who know the media or broth for bacterial contamination Q.A
> of dialysis water or distilled water.
> Simon Lee
>>