gary,
you may want to check medline for "mutS" mutants of e.coli. if i
remember correctly, growing your plasmids in that type of strain
increases the probability for random mutations.
regards,
helen
In article <Pine.GSO.4.10.10011141202430.770-
100000 at konichiwa.cc.columbia.edu>,
"Gary G. Wilson" <ggw5 at columbia.edu> wrote:
> I'm new to the field and am looking for references to definitive
articles
> on methods for using mutational strains of bacteria to create random
> mutations in a plasmid. Can anyone point me in the right direction.
>> Gary G Wilson
>>
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