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Sputum for C&S

lamb L.A.M.Buisman at cable.A2000.nl
Wed May 31 14:20:31 EST 2000

I also wonder if the Dutch are the only ones.
I have colleagues from Germany, South-Africa, Bosnia. None of them ever said we
were doing something odd.
The other "foreign" colleagues were trained here so they don't count.

Dangerous, it would seem to be. Strange really, in our lab we are very much aware
of dangerous procedures. But this is the standard procedure in this country.
The washing is done cautiously, if  rougher washing is needed there is no question
of splashing. I have never (in 31 years) encountered lab. infections, no more colds
than normal, and no TB. I know, that is no valid reason to say it is absolutely


Dilworth wrote:

> Wow!  Quite an involved procedure.  Here in the US, most sputum specimens
> (coughed, not tracheal aspirates or any type of suction specimens) are gram
> stained directly from the specimen container.   If there are >25 epis/low power
> field, the specimen is rejected then and there.  Seems pretty dangerous to
> openly be messing with sputum, even under a hood.  I've never worked anywhere
> that did the wash.  Anyone else out there washing their sputum specimens like
> this?  Just wondering.  I have no doubt it works, but in relation to our
> extremely heavy workload, I can't imagine taking all this time with one
> specimen.
> Judy Dilworth, M.T. (ASCP)
> Microbiology

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