I have a colleague who is looking for a transposon that carries
antibiotic resistance and that can be used for the insertional
inactivation of a carbohydrase gene of a Gram negative Flavobacterium.
Does anyone have, or know where he can get a Tn5 transposon, or a
similar kind of transposon that could be used for gene inactivation,
which carries an antibiotic resistance gene for any of the following:
chloramphenicol, erythromycin or ampicillin?
Please send replies to my colleague: david.humphry at sunderland.ac.uk
Many thanks in advance for your help!
Dr Dean Harrington
Lecturer in Molecular Microbiology
School of Sciences
University of Sunderland
Tel: +44 (0)191 515 2995
FAX: +44 (0)191 515 3747
e-mail: dean.harrington at sunderland.ac.uk