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I need help

David Lawton dhlawton at clara.co.uk
Fri Mar 24 17:57:41 EST 2000

The brain's not working this evening - I do know what oligotrophic bacteria
are, just not used to using the posh name.
Yeast extract agar is recommended for water samples in the UK.
For general air samples tryptone soya agar is often recommended, however for
our routine use we find no trouble using blood agar. Depends of cause why
you're taking the air samples

I still wouldn't call blood agar isn't a selective medium though.

A medium that doesn't grow an organism as well as another one doesn't mean
it's selective.

To my mind a selective media is something where an ingredient is added to
make it selective for a specific organism or group of organisms.
If you added, for example, nalidixic acid and colistin then you have a
selective medium.

David Lawton

"lamb" <L.A.M.Buisman at cable.A2000.nl> wrote in message
news:38DBC8FB.8DCCDF6A at cable.A2000.nl...
> David Lawton wrote:
> > As I know nothing about oligtrophic bugs I can't comment,
> > but being highly pedantic, for air samples wouldn't blood agar be
> > a non - selective medium
> > especially when compared with nutrient agar.
> For culturing watersamples nutrient agar or R2A is recommended. We find a
> 100-1000 fold increase in colony count compared to blood agar.
> Air samples can show fantastic amounts of growth on bloodagar. Usually the
> lot gets overgrown with molds.
> Loes

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