If it wants to attach, let it. Increase the surface area (beads,
parallel plates). Grow to a steady-state biofilm. Get yields by
measuring biomass of the effluent. Much more difficult, but truer to
the organism.
bo wrote:
>> Hi,
>> I'm currently growing Chl. tepidum (an anaerobic green sulfur bacterium) in
> continuous culture at a dilution rates of 0.15 h-1 and using the Wahlund's
> medium (Wahlund et al., 1991; Arch. Microbiol. 156:81-90). The main problem
> is that it heavily attachs on the vessel walls, which makes impossible
> further calculations on light intensity available, cell yields, etc. I know
> that the problem is not new but I don't know for the moment how to avoid it.
> If anybody knows how to solve it (maybe it is enough with a slight
> modification of the growth medium?), please, don't hesitate to contact me.
>> Carlos
> _______________________________________
> Carles M. Borrego
> Lab. Microbiology, Institute of Aquatic Ecology
> University of Girona
> Campus de Montilivi, E-17071, Girona (Spain)
> Phone: +34 972 418175
> FAX: +34 972 418150
> _______________________________________
--
~DBH
Technical writing, literature search, and data analysis at the interface
of chemistry and biology.
davidbhedrick at icx.net
David B. Hedrick
P.O. Box 16082
Knoxville, TN 37996
