I think all I am asking folks to do is help out, encourage and point out when
some bad advice comes across the NG. And do so in a way that doesn't offend the
misinformed (OK, sorry I called everyone voodoo priests, but better a priest
than a journeyman ;>) No-one seems to notice the problem with advising students
to use LB either (see earliest posts). I think the concerns others have noted
regarding pathogenic bacteria are warranted if we keep suggesting using this
media: 10 g NaCl per liter is exactly what a pathogen needs! Think of it as a
selective agent for isolating pathogens only. No one seems to pick up on this
nugget and this is but one of several times I have seen the suggestion. Think
high school projects scares you? see below:
> Question: I am 7th grade student doing an experiment with environmental bacteria. I am
> using Luria Broth Agar. I need to know what nutrients are used in this agar.
> name deleted
>> Answer:
> If you simply need the recipe, I can provide that for you.
> However, Luria Broth is a rich medium, which means its content is
> largely undefined as far as the presence and concentrations of
> various nutrients. The recipe for 1 liter:
>> 10 g Bacot-tryptone
> 5 g Bacto-yeast extract
> 10 g NaCl
> pH 7.0
>
Perhaps try R2A agar or weakened TSAYE, potato-flake agar for fungi.
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