It's actually a 9th grade honors class. I'm 15, that's about everyone's age. My
e-mail should be working since I sent an e-mail to myself and got it back.
Thanks for the info, I'll try to get to a university, although there aren't any
real close.
thanks
Riffi
Errol wrote:
> I tried emailing you but it didn't go through. So what level is 9th
> Grade, what's the average age at this level? For text books I didn't
> think a high school library would be of much help. If you have a
> university in your town you could go there and read their books on
> microbiology. One book I used as an undergraduate was 'Biology of
> microorganisms' by Brock and Madigan, this maybe quite helpful for
> you, but you should be able to search the university catalogue for
> more.
>> On Thu, 16 Mar 2000 02:54:23 GMT, Riffi <riffi at cyberspace.org> wrote:
>> >To answer your questions, I'm in 9th grade Biology. This is sort of an
> >optional extra credit assignment. Although the teacher is will help some
> >what by setting it up in the lab, we have to get all research ourself, and
> >do all the work ourself. The textbook does not cover this area, and the
> >school library was not very helpful. And yes, my education is the american
> >type (pennsylvania actually).
> >Thanks for the help
> >Riffi
> >Errol wrote:
> >
> >> Judging by the question I would say that you probably couldn't follow
> >> this answer.
> >>
> >> >Best bet is some flavor of growth media like LB.
> >>
> >> LB stands for Luria broth, it's a basic solution that allows the
> >> growth of most micro-organisms. I would suggest that if you use this
> >> that you make it into an agar plate, this is done by adding some agar
> >> into the solution and poured into a petri dish, when it cools it
> >> solidifies and you can see colonies growing on the surface, then from
> >> this you can look at the colonies for their shape colour and other
> >> physical features, and look at a sample of each under a microscope to
> >> look at the cells.
> >>
> >> >Depends upon the strain of bacteria.
> >> >That depends on where you grow it. If you put a plate of LB in your
> >> >bed room, bathroom, dining room, outside, etc bacteria will begin to
> >> >grow on it as they are everywhere. If you are doing this in a 'clean'
> >> >lab setting you will need to innoculate the media with bacteria.
> >>
> >> If you expose your plates to the air, then you should incubate the
> >> plates after exposure at about 25 degrees celcius as this is
> >> considered to be room temperature. This will allow microbes in the
> >> room to grow. I would grow them overnight and see what the growth is
> >> like, you don't want growth of one organism to cover the entire plate
> >> so you can't see any other colonies. If there isn't much growth then
> >> leave it another night. You'll want to seal the lids on the plate as
> >> well, as if anything pathogenic grows you don't want to expose
> >> yourself to it and make yourself sick.
> >>
> >> >First you will need to identify the different species of bacteria you
> >> >have. There SOPs for this and commercial kits you can buy as well.
> >> >Once you have that, count up the number of colonies each type of
> >> >bacteria has.
> >>
> >> You probably won't have access to these sorts of kits and may have
> >> some difficulty buying them, but try to get them anyway.
> >>
> >> >>How do I figure out the kinds that grew?
> >> >
> >> >See above.
> >>
> >> You could also look at them under the microscope and examine the
> >> individual cells and try to identify them this way, along with the
> >> physical features of the colony. I would suggest that you try to
> >> identify them this was as you will get more out of the experience than
> >> just reading what the testing kit tells you.
> >>
> >> >>Are they any books or websites that can help me out?
> >> >
> >> >O.K., a wuick search on 'ANY' search engine will bring up plenty of
> >> >sites. Checking in at you school's library will reveal plenty of
> >> >microbiology texts/journals. Have you tried your class text book?!?!?
> >> >
> >> >Just out of curiosity what level class is this that give syou such
> >> >pitiful instruction? Anyone worried that you might infect yourself
> >> >with something that you would be better off without?
> >> >
> >> I would like to know you class level as well and your age in case your
> >> education system is the american type. I'm also not sure what sort of
> >> microbiology text and journals your school would have. You should
> >> consult your science teacher as she/he may have some books and should
> >> explain about making up culture media.
> >
> >
> >
