On Mon, 13 Mar 2000, Alan S. Wicks wrote:
> Greetings,
>> Will someone please give me a (or a few) good references on
> cryoprotectants for bacteria.
>> Thanks,
> Alan S. Wicks
> Kennewick, WA
>
Hi Alan,
Why not check out PubMed <http://www.ncbi.nlm.nih.gov> using
"(cryoprotect* AND (bacteria OR e. coli))"
or try the multi-search engine Dogpile <http://www.dogpile.com>
I did the same with trehalose-my favorite protectant, since you
can also freeze dry with it and received some of the following
references.
Hope this helps,
Brad Turner
****************************************************************
Bradley Turner
Beth Israel Deaconess
Medical Center
Harvard Medical School 617-667-1215 phone
Division of Gastroenterology 617-667-2767 fax
Room Dana 536 bsturner at biosun.harvard.edu
330 Brookline Avenue bturner at caregroup.harvard.edu
Boston, MA 02215 turner at sprcore.caregroup.harvard.edu
****************************************************************
REFERENCES-----------------------------------------------------
1: Israeli E, Shaffer BT, Lighthart B.
Protection of freeze-dried Escherichia coli by trehalose upon exposure
to environmental conditions.
Cryobiology. 1993 Oct;30(5):519-23.
PMID: 8252918; UI: 94074358
2: Leslie SB, Israeli E, Lighthart B, Crowe JH, Crowe LM.
Trehalose and sucrose protect both membranes and proteins in intact
bacteria during drying.
Appl Environ Microbiol. 1995 Oct;61(10):3592-7.
PMID: 7486995; UI: 96090613
3: Diniz-Mendes L, Bernardes E, de Araujo PS, Panek AD, Paschoalin VM.
Preservation of frozen yeast cells by trehalose.
Biotechnol Bioeng. 1999 Dec 5;65(5):572-8.
PMID: 10516583; UI: 99447455
4: Lodato P, Se govia de Huergo M, Buera MP.
Viability and thermal stability of a strain of Saccharomyces cerevisiae
freeze-dried in different sugar and polymer matrices.
Appl Microbiol Biotechnol. 1999 Aug;52(2):215-20.
PMID: 10499261; UI: 99429076
5: Klein W, Boos W.
Induction of the lambda receptor is essential for effective uptake of
trehalose in Escherichia coli.
J Bacteriol. 1993 Mar;175(6):1682-6.
PMID: 8449875; UI: 93194793
6: Welsh DT, Herbert RA.
Osmotically induced intracellular trehalose, but not glycine betaine
accumulation promotes desiccation tolerance in Escherichia coli.
FEMS Microbiol Lett. 1999 May 1;174(1):57-63.
PMID: 10234822; UI: 99251130
7: Crowe LM, Crowe JH, Rudolph A, Womersley C, Appel L.
Preservation of freeze-dried liposomes by trehalose.
Arch Biochem Biophys. 1985 Oct;242(1):240-7.
PMID: 4051504; UI: 86024285
8: Eroglu A, Russo MJ, Bieganski R, Fowler A, Cheley S, Bayley H, Toner
M.
Intracellular trehalose improves the survival of cryopreserved mammalian
cells.
Nat Biotechnol. 2000 Feb;18(2):163-7.
PMID: 10657121; UI: 20124246
9: Hino A, Mihara K, Nakashima K, Takano H.
Trehalose levels and survival ratio of freeze-tolerant versus
freeze-sensitive yeasts.
Appl Environ Microbiol. 1990 May;56(5):1386-91.
PMID: 2339891; UI: 90253196
10: Yoshinaga K, Yoshioka H, Kurosaki H, Hirasawa M, Uritani M, Hasegawa
K.
Protection by trehalose of DNA from radiation damage.
Biosci Biotechnol Biochem. 1997 Jan;61(1):160-1.
PMID: 9028044; UI: 97179808
11: Guo N, Puhlev I, Brown DR, Mansbridge J, Levine F.
Trehalose expression confers desiccation tolerance on human cells.
Nat Biotechnol. 2000 Feb;18(2):168-71.
PMID: 10657122; UI: 20124247
12: Bieganski RM, Fowler A, Morgan JR, Toner M.
Stabilization of active recombinant retroviruses in an amorphous dry
state with trehalose.
Biotechnol Prog. 1998 Jul-Aug;14(4):615-20.
PMID: 9694684; UI: 98361813
13: Crowe LM, Reid DS, Crowe JH.
Is trehalose special for preserving dry biomaterials?
Biophys J. 1996 Oct;71(4):2087-93.
PMID: 8889183; UI: 97044112
14: Rossi S, Buera MP, Moreno S, Chirife J.
Stabilization of the restriction enzyme EcoRI dried with trehalose and
other selected glass-forming solutes.
Biotechnol Prog. 1997 Sep-Oct;13(5):609-16.
PMID: 9336981; UI: 97478068
15: Uritani M, Takai M, Yoshinaga K.
Protective effect of disaccharides on restriction endonucleases during
drying under vacuum.
J Biochem (Tokyo). 1995 Apr;117(4):774-9.
PMID: 7592538; UI: 96070172
16: Colaco C, Sen S, Thangavelu M, Pinder S, Roser B.
Extraordinary stability of enzymes dried in trehalose: simplified
molecular biology.
Biotechnology (N Y). 1992 Sep;10(9):1007-11.
PMID: 1369408; UI: 93098914
17: Sola-Penna M, Meyer-Fernandes JR.
Stabilization against thermal inactivation promoted by sugars on enzyme
structure and function: why is trehalose more effective than other
sugars?
Arch Biochem Biophys. 1998 Dec 1;360(1):10-4.
PMID: 9826423; UI: 99045380
18: Hottiger T, De Virgilio C, Hall MN, Boller T, Wiemken A.
The role of trehalose synthesis for the acquisition of thermotolerance
in yeast.
II. Physiological concentrations of trehalose increase the thermal
stability of proteins in vitro.
Eur J Biochem. 1994 Jan 15;219(1-2):187-93.
PMID: 8306985; UI: 94139691
19: Draber P, Draberova E, Novakova M.
Stability of monoclonal IgM antibodies freeze-dried in the presence of
trehalose.
J Immunol Methods. 1995 Apr 12;181(1):37-43.
PMID: 7730665; UI: 95248135
20: Miller DP, de Pablo JJ, Corti H.
Thermophysical properties of trehalose and its concentrated aqueous
solutions.
Pharm Res. 1997 May;14(5):578-90.
PMID: 9165527; UI: 97308301
21: Kuleshova LL, MacFarlane DR, Trounson AO, Shaw JM.
Sugars exert a major influence on the vitrification properties of
ethylene
glycol-based solutions and have low toxicity to embryos and oocytes.
Cryobiology. 1999 Mar;38(2):119-30.
PMID: 10191035; UI: 99208625
22: Crowe JH, Carpenter JF, Crowe LM.
The role of vitrification in anhydrobiosis.
Annu Rev Physiol. 1998;60:73-103. Review.
PMID: 9558455; UI: 98219146
23: Paiva CL, Panek AD.
Biotechnological applications of the disaccharide trehalose.
Biotechnol Annu Rev. 1996;2:293-314. Review.
PMID: 9704101; UI: 98369724
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