Storage in liquid nitrogen is probably the best long term method, but not the
only or cheapest, for preserving filamentous fungi.
A cheaper alternative can be storage as water cultures.
Try the references below and look out for the CMI book; The preservation and
maintenance of fungi by D. Smith & A.H.S. Onions. I have the 1983 edition
but I'm sure there is a revised edition.
Challen, M.P. & Elliott, T.J. (1986) Polypropylene straw ampoules for the
storage of micro-organisms in liquid nitrogen. Journal of Microbiological
Methods 5, 11-23.
Boeswinkel, H.J. (1976) Storage of fungal cultures in water. Transactions of
British Mycological Society 66, 183-185.
Jones R.J. et. al. (1991) A minaturised system for storage of fungal cultures
in water. Mycologist 5, 184-186.
Good luck!
Mike
jayakumar wrote:
> HI
> This may sound silly. But can anyone, tell me a good method for long
> storage of fungal cultures like they do for bacteria by glycerol storage.
> secondly, a good protocol for fungal staining and visualisation under a
> light microscrope.
> thanking you in advance
> cheers
> jayakumar
