Some sources of information:
"Fungi and Food Spoilage" by John Pitt & Ailsa Hocking. I have the first
edition (1985 Academic Press) which has a page and a half on storage methods
including freezer, lyophilising, liquid nitrogen. A second edition of this
book is now in print.
The CBS has some publications (some downloadable) on mold storage. Go to
www.cbs.knaw.nl.
The Commonwealth Agricultural Bureau offers a manual on fungal maintenance:
The Preservation and Maintenance of Living Fungi by D. Smith and A.H.S.
Onions (1994) 122 pages ISBN 0 85198 902 0. Go to
www.cabi.org/bioscience/mycopub.htm#hand . I think you may have to buy this
one.
One method not mentioned by Pitt & Hocking is storage in sterile water. I
once worked with someone who kept his mycorrhizal stock cultures this way.
I used the sterile oil method years ago and it is indeed messy.
Common fungi in the genera Aspergillus, Penicillium, Cladosporium will keep
a long time just on agar slants which are sealed against dessication.
--
Austin Reade, PhD, RSM(CCM)
Reade BioSciences Inc phone (902)423-8369
1136 Cartaret Street fax (902)423-8313
Halifax, NS, B3H 3P3 Canada email rbs at hfx.andara.com
jayakumar <jakku at usaf.org> wrote in message
news:8j7l80$n4o$1 at diana.bcn.ttd.net...
> HI
> This may sound silly. But can anyone, tell me a good method for long
> storage of fungal cultures like they do for bacteria by glycerol storage.
> secondly, a good protocol for fungal staining and visualisation under a
> light microscrope.
> thanking you in advance
> cheers
> jayakumar
>>>>
