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Western blotting question

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Mon Jun 26 03:59:41 EST 2000


How about Chemiluminescence? This is much more sensitive.

Suzanne Hermeling wrote:

> Hi
>
> I have a small question about western blotting. I have a substrate that
> reacts with GARPO to give a soluble blue product (i.e. TMB-dichloride).
> I have always used this in an ELISA. However I want to run Western Blots
> as well.
>
> Does anyone know whether I can use the same substrate or do I have to
> get a product that results in a non-soluble product?
> I have searched on the web-page of Pierce and they say that the soluble
> substrate is for ELISA and they don't say anything about Western
> Blotting with this substrate.
>
> Can anyone help me??
>
> Suzanne

--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland

srohrer at immv dot unizh dot ch







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