How about Chemiluminescence? This is much more sensitive.
Suzanne Hermeling wrote:
> Hi
>> I have a small question about western blotting. I have a substrate that
> reacts with GARPO to give a soluble blue product (i.e. TMB-dichloride).
> I have always used this in an ELISA. However I want to run Western Blots
> as well.
>> Does anyone know whether I can use the same substrate or do I have to
> get a product that results in a non-soluble product?
> I have searched on the web-page of Pierce and they say that the soluble
> substrate is for ELISA and they don't say anything about Western
> Blotting with this substrate.
>> Can anyone help me??
>> Suzanne
--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland
srohrer at immv dot unizh dot ch
