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T4 phage transduction

s97000467 at student.usp.ac.fj s97000467 at student.usp.ac.fj
Tue Jun 13 16:59:15 EST 2000








In article <39360029.E41ED59E at tamu.edu>, Jim Hu <jimhu at tamu.edu> writes:
> Rafael ,
> 
> You are correct. If Ned is still following this thread: Generalized
> transduction occurs with phages that use headful packaging mechanisms,
> these include P1, P22 and certain T4 mutants.  Wild-type T4 is not a good
> generalized transducing phage, since destruction of the host genome
> happens as part of the normal T4 life cycle.  T4 has advantages over P1
> because it packages a larger DNA segment into transducing particles than
> P1.
> 
> As for protocols, I haven't done T4 transduction but I found a fairly
> recent reference that might be a good place to start.
> 
> Lin H. Black LW. DNA requirements in vivo for phage T4 packaging.
> Virology. 242(1):118-27, 1998 Mar 1.
> 
> Hope this helps.
> 
> Jim Hu
> Dept. of Biochemistry and Biophysics
> Texas A&M Univ.
> 
> Rafael Maldonado wrote:
> 
>> Ned Mantei wrote:
>> >
>> > T4 grows lytically, so no transduction.
>> >
>>
>> Ned, thanks for your message.
>>
>> T4 CAN make transduction. P1 is also a lytic phage, and I have used it
>> hundreds of times, together with thousands of other scientits, for
>> rotinary transduction in coli and Klebsiella.
>>
>> -Rafa
>>
>> --
>> Rafael Maldonado
>> Divison of Genetics
>> University of Alicante (Spain)
> 






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