In article <39360029.E41ED59E at tamu.edu>, Jim Hu <jimhu at tamu.edu> writes:
> Rafael ,
>> You are correct. If Ned is still following this thread: Generalized
> transduction occurs with phages that use headful packaging mechanisms,
> these include P1, P22 and certain T4 mutants. Wild-type T4 is not a good
> generalized transducing phage, since destruction of the host genome
> happens as part of the normal T4 life cycle. T4 has advantages over P1
> because it packages a larger DNA segment into transducing particles than
> P1.
>> As for protocols, I haven't done T4 transduction but I found a fairly
> recent reference that might be a good place to start.
>> Lin H. Black LW. DNA requirements in vivo for phage T4 packaging.
> Virology. 242(1):118-27, 1998 Mar 1.
>> Hope this helps.
>> Jim Hu
> Dept. of Biochemistry and Biophysics
> Texas A&M Univ.
>> Rafael Maldonado wrote:
>>> Ned Mantei wrote:
>> >
>> > T4 grows lytically, so no transduction.
>> >
>>>> Ned, thanks for your message.
>>>> T4 CAN make transduction. P1 is also a lytic phage, and I have used it
>> hundreds of times, together with thousands of other scientits, for
>> rotinary transduction in coli and Klebsiella.
>>>> -Rafa
>>>> --
>> Rafael Maldonado
>> Divison of Genetics
>> University of Alicante (Spain)
>
