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Western blotting question

Jay Mone jaymone at paonline.com
Sun Jun 11 10:42:22 EST 2000

Assuming that GARPO is a peroxidase conjugate, try diaminobenzidine as the
substrate for your western blots.  Add 100 mg of DAB to 100 mls of PBS.
Just before use, add 75 ul of 30% hydrogen peroxide.  After bands develop,
the reaction can  be stopped by flooding the blots with water.

Jay Mone'


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