"Lynn" <kees.lyn at xtra.co.nz> wrote in message
news:39364780.F9C at xtra.co.nz...
> lamb wrote:
> We use TSA or R2A for total counts, Cled for coliforms. 0.1 ml. per
> > Loes
>>> R2A was on my list, What is Cled? We currently do a lot of VRBA plates
> for coliforms but I was going to try a broth for MPN on the water
> samples, esp ones with low counts.
> Cheers Lynn
CLED - Cystine Lactose Electrolyte Deficient
It's probably more commonly used for urines.
IMHO 0.1ml wouldn't give a sufficient sample size for this type of test.
Counts from bore holes could well (no pun intended) be 0 in 100 ml
For E. coli & Coliforms we use membrane filtration and membrane lauryl
For total counts yeast extract agar at 37 & 22C
If you are going to do MPN we used to use minerals modified glutamate medium