Mycology/PCR

[Dirk M ü ller]

There has been done some work on specific primer located in the ITS and the
rDNA regions for differentiation of fungi. The problem is that you have to
develope special primers for each genus or species. Only thing is to use the
ITS primers from White et al. These primers are developed in Saccharomyces
cerevisiae and amplify most fungal DNA too. But you can't differentiate with
these primers.


Dipl.-Ing. Dirk Müller
University of Technology Berlin
Dept. of Microbiology and Genetics, TIB 4/4-1
Gustav-Meyer-Allee 25
D-13355 Berlin, Germany

Phone: + 49-30-314-72271
Fax: + 49-30-314-72922
e-mail: D.Mueller at lb.TU-Berlin.DE
http://www.tu-berlin.de/biotec/mibi

> Von: Dale w Griffin <dgriffin at usgs.gov>
> Organisation: USGS
> Newsgroups: bionet.microbiology
> Datum: Wed, 5 Jul 2000 20:34:17 GMT
> Betreff: Mycology/PCR
> 
> Anyone know of a good reliable universal primer set that can be used for
> the identification of various types of fungi?
> 
> Thanks,
> 
> Dale Griffin
>