IUBio

NEED PROTOCOL: PCRing genes from E.Coli

Rogier rogierNOroSPAM at bio.vu.nl.invalid
Tue Jul 11 15:08:00 EST 2000


Hiranya Sankar Roychowdhury wrote:

>.....and it's copy number hundreds of times lower.
>>huh?!

plasmid: hundreds of copies per cell. sometimes well over a
thousand.
chromosome: depending on where in cell cycle, usually 1 or 2
copies. more copies at exotic growth conditions, but far far far
below the hundreds of copies of cloning vectors like pBluescript
etc.

Rogier


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