IUBio

gram pos DNA extraction

Neil McKenna nmckenna at ottawa.com
Mon Jul 10 10:53:11 EST 2000


Hello Peter,

I don't know how high quality DNA you are looking for, but I am
attaching a WordPerfect file of simple and quick genomic DNA extraction
procedures for both gram positive and negatives. I routinely use DNA
from this procedure for cloning, Southern blotting and PCR, but I have
no experience with other techniques. Hopefully it works for you!

Good luck
Neil McKenna
Department of Microbiology and Immunology
University of Ottawa, Ottawa, Canada

Peter Voth wrote:
> 
> Hello Group,
>         I am looking for a good method to extract some good quality DNA
> from a gram pos. bacteria, clavibacter michiganense pv michiganense. If
> anyone can help me, thanks a bunch.
> Peter Voth
> UC Davis






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