Hi Philippe,
I think the best way for you to obtain this data - as well as a good
standard curve - is to use p-nitrophenol as a reference standard, at the
same conditions you're using in your assays.
(Of course, remember to control again your pH after addition of
p-nitrophenol, since it could change at high concentration, and it would
alter the e.c.)
Have good work!
-Roberto
----- Original Message -----
From: philippe giammarinaro <philippe.giammarinaro at adelaide.edu.au>
To: <microbio at hgmp.mrc.ac.uk>
Cc: <bionet.biology at adelaide.edu.au>
Sent: Monday, December 18, 2000 11:27 PM
Subject: help need of extinction coef of p nitrophenyl
> hey,
> I m using p-nitrophenyl
> alpha-d-glucopyranoside to quantify the
> activity of
> alpha glucosidase i have in my samples, but i
> miss an important data: What
> is the millimolar extinction coefficient of
> p-nitrophenyl at 410nm et at
> 37o C and pH 6.6?
> Thank in adavance for your respons.
> Philippe Giammarinaro
>
---
