On Sat, 2 Dec 2000, Michael Witty wrote:
> Dear Peter, I use E. coli only, so I could just dip the probe in dilute
> ethanol before use. Actually nothing more that washing up liquid might
> be OK! What do you do at the Department of Microbiology?
> Mike
>> On Sat, 2 Dec 2000, Peter C. Giardina, Ph.D. wrote:
>> > On Sat, 2 Dec 2000, Michael Witty wrote:
> >
> > > Dear All, I am convinced there must be a better way of finding the log
> > > phase of growth (for example of a conical flask of E. coli) than
> > > repeatedly taking 1ml samples and putting them in a spec.
> > >
> > > My ambition is to have a cheap detector with a probe consisting of a
> > > light source and photoresistor which can be dangled into my flask and
> > > record data continuously.
> > >
> > > But I am a biologist not an electrical engineer. Does anyone share
> > > my ambition? Or know how I can make this happen? Mike.
> > >
> > >
> >
> > Hmmm... Not sure how you'd sterilize the thing by a convenient method
> > without destroying it. And some folks have to shake their cultures pretty
> > hard. It would have to be one tough little device.
> >
> > Peter
>
I'm a bacteriologist. I work with pathogenic Neisseria species.
Regarding the probe device, you may try using side-arm culture flasks
which can be inserted directly into a spec. It isn't continuous
monitoring, but better than opening your flask to take samples at time
points....reduced the risk of contamination.
Good luck!
Peter
---------------------------------------------------------------------
Peter C. Giardina, Ph.D.
Univ. Iowa Dept. Microbiology
BSB 3-401, 51 Newton Rd.
Iowa City, IA 52242
voice: (319) 353-5654
FAX: (319) 335-9006
<peter-giardina at uiowa.edu>
---------------------------------------------------------------------
