Before you use BLAST, you should familiarize yourself with what it does. It
is almost a given that BLASTX and BLASTN will show different results since
the two do different things. Which one you use depends upon your input
data; your knowledge about the query seq., what you expect, the source of
the query, etc., ar to be considered.
At 01:48 PM 8/27/00 GMT, parvaizqazi at my-deja.com wrote:
>I sequenced a pcr amplified fragment and analysed this sequence through
>BLASTX and BLASTn programmes.Both of these show different results.Which
>one should I stick to or what is the right programme, I must follow.
>>>Sent via Deja.com http://www.deja.com/>Before you buy.
>>>Dr. Hiranya Sankar Roychowdhury
Dept. of Molecular Biology
PO Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003
Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu