Hi, I did this at the last place I worked. Basically I cut the band out of
the polyacrlyamide gel put it in water (I think about 50ul), heated to
95c for a couple of minutes, and then 2ul used for the next pcr as
normal. Worked really well , and was a lot easier than other methods that
were suggested. If you want I can find out the exact details of the
protocol (above was from memory) but I don't have them at hand at the
moment. Hope this helps,
On 16 Aug 2000, Larsen, Lise wrote:
> How do you extract DNA from a polyacrylamide gel?
> I run DGGE gels and need to excise the bands for PCR reamplification and=
> sequencing. I've tried cut out bands and freeze/thaw them =D73 in water =
> didn't get=20
> any product after the following PCR. Please, let me know if you know of a
> method to recover the DNA.
> Thank you
> Lise Larsen