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very quickly its made up of Beef extract 1g/L Yeast extract 2g/L Peptone 5g/L NaCl 5g/L that's for broth, for agar just add 15g agar/L Best of luck - Eoin Eoin Brodie Dept Industrial Microbiology University College Dublin Belfield Dublin 4 IRELAND Tel. +353 1 706 1574 Fax. +353 1 706 1183 ---