In article <39074642.4724D1ED at aol.com>, Arkangel67 at aol.com wrote:
> I need to know the ingredients in nutrient agar/broth for an elementary
> school science project. Is it any different from LB or Terrific broth?
> Thanks!
>
I should know this off the top of my head but sorry....I'm not at my
office with all the reference books.
however,
I think Nutrient Agar/Broth contains peptone and beef extract...maybe a
small amount of dextrose, but I'm not sure of that.
Most likely, LB or Terrific would support the organisms you plan to use
for an elementary school project. Nutrient Ag/Br is richer, but the
other two would work for most of the Enterobacteriaceae, Pseudomonads,
Staph and Micrococci, and Bacillus sp. LB contains peptone, NaCl, and
yeast extract (quantities are different depending on which LB you have,
Lennox or Miller). LB is primarily used to support good growth of
plasmid-carrying E coli's used in many molecular biology labs.
Martin Gross
Supervisor, Media Prep Lab
American Type Culture Collection
mgross at atcc.org
