Is manganese a divalent cation as is calcuim and magnesium? if so, try a
little EDTA (0.5mM to 5 mM) and see if that doesnt work. If I am confused,
and it is not a divalent cation, the please ignore this post !
cheers,
Megan
PS. when making up EDTA as a stock, you will have to pH it to get it to go
into solution. EDTA is made up as a 0.5 M solution, but it will not go into
solution without pHing to about 7.5 or 8 or so. It will not (to my
knowledge) go into solution at higher concentrations.
GIBSON wrote in message <7vc698$h8f$1 at ucsnew1.ncl.ac.uk>...
>I am currently doing my final year research project, for my BSc. I am
>looking into the effect of manganese on sporulation in Bacillus subtilis.
>>Does anyone know any compound that would specifically interact with free Mn
>and remove it from solution??
>>Thanks,
>>Kevin Gibson
>>
