Hi Anson
The heating step used to be needed to inactivate DNases that
co-purify with RNaseA. Nowadays most commercially available RNaseA is
guaranteed free of DNase activity and heating is not advised.
Happy digesting!
Alastair
Alastair Hamilton
Institute of Aquaculture
University of Stirling
Scotland FK9 4LA
On Mon, 18 Oct 1999, Anson Li wrote:
> Hi Microbio people!
>> RNase is prepared by making the appropriate concentration in buffer,
> heating it to 100 degrees for 15 minutes, and then allowed to cool slowly
> to room temperature. It is stored at -20 degrees celsius.
>> Is the heating process there to get rid of the oxygen? But it doesn't
> seem to make sense unless RNase was inhibited by oxygen???
> And the storing of the protein in -20 degrees celsius is there because
> RNase degrades slowly at room temperature??? Or is there
> another reason?
>> Your input is appreciated.
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