Staining Staphylococcus

Bob/Judy Dilworth dilworth at megsinet.net
Tue Oct 19 20:35:22 EST 1999

The way I do bacterial stains with Acridine orange is this:

1.  Air dry the slide of whatever it is you want to stain.
2.  Fix the stain by flooding it with acetone-alcohol (gram stain
decolorizer - 50% acetone/50% of 100% ETOH if you have it, otherwise
use 95% ETOH) for two minutes.
3.  Tip this off and flood with Acridine orange for two minutes.
4.  Rinse with tap water and let air dry - then read under fluorescent

Hope this helps.  Our lab also uses Acridine orange to stain for
trichomonas with some modification of the above technique.

Judy Dilworth, M.T. (ASCP)
Microbiology 25 years

reddy kesav wrote:
> I am trying to stain Staphylococcus aureus with Acridine Orange to count
> the bacteria which adhere under flow.
> Could anyone suggest a suitable protocol for Acridine
> Orange?

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