Hi, Ricki
As a clinical microbiologist, I do not find L.
mesenteroides too often, but when I do it grows quite
easily on sheep-blood agar or choc-agar incubated at
37ºC-24h in normal atmosphere. Maybe that it takes
more than 24h to grow at 28ºC.Take it easy.(Or maybe
it doesn´t like tomato at all....:)).
--- Frederik Boernke
<boernke at nospam.ipk-gatersleben.de> wrote:
> Hi,
>> I'd would like to cultivate L. mesenteroides in
> order to prepare
> DNA. I obtained some freeze-dried stuff from the
> DSMZ which I
> plated onto acidic tomato medium. However, I can not
> observe any
> growth after 24 h at 28 deg.. My textbook knowledge
> tells me that
> those bugs do actually ferment, but should be
> aerotolerant.
> Any hints are appreciated.
>> Ricky
>>*******************************************************************
>> Frederik Boernke
> Research Group of Molecular Plant Physiology
> Institute for Plant Genetics and Crop Plant Research
> (IPK)
> Corrensstr. 3
> 06466 Gatersleben
> Tel. 039482 -5 321
> Fax. 039482 -5 515
>http://www.ipk-gatersleben.de>
=====
__________________________________________________
Do You Yahoo!?
Bid and sell for free at http://auctions.yahoo.com
---
