Washing E.coli cells/ osmotic pressure determination

Olivier ofb at wanadoo.fr
Tue Oct 12 13:35:01 EST 1999

We are currently washing E. coli cells with a water solution containing 9 g/
l of NaCl. However, it seems that we have some cell lysis.
I was wondering if it was an osmotic pressure problem? Do you know a simple
methode to measure an osmotic pressure? Do you know if I must modify the
concentration of NaCl that i'm using.

Thank you for your help.
Olivier Favre-Bulle
oliver.favre-bulle at wanadoo.fr

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