We are currently washing E. coli cells with a water solution containing 9 g/
l of NaCl. However, it seems that we have some cell lysis.
I was wondering if it was an osmotic pressure problem? Do you know a simple
methode to measure an osmotic pressure? Do you know if I must modify the
concentration of NaCl that i'm using.
Thank you for your help.
--
Olivier Favre-Bulle
oliver.favre-bulle at wanadoo.fr
