New system for isolation of E Coli 0157 from IDG

michelle kerr kerrmichel at HOTMAIL.COM
Thu Oct 7 12:42:57 EST 1999

How expensive will the Captivat0 be?

>From: "Liz Middleton" <liz.middleton at lewis-associates.co.uk>
>To: microbio at net.bio.net
>Subject: New system for isolation of E Coli 0157 from IDG
>Date: Thu, 07 Oct 1999 11:02:47 +0100
>IDG has introduced Captivate O157, an alternative system for the isolation
>of E.coli O157. The system comprises specific antibody-coated magnetic 
>for the isolation of E.coli O157 from food, animal feed, beverages,
>pharmaceutical or environmental samples.
>E.coli O157:H7 is a potentially life-threatening gastrointestinal pathogen.
>The majority of infections are foodborne and associated with ground beef,
>hamburgers and raw milk. Conventional isolation methods can give rise to
>false negative results due to the problem of competing flora obscuring
>E.coli O157 colonies.
>The recommended protocol employs enrichment in Modified Tryptone Soy Broth
>(MTSB) followed by separation with Captivate, prior to sub-culture of the
>O157-bead complex on Sorbitol MacConkey Agar supplemented with cefixime and
>potassium tellurite (CT-SMAC). E.coli O157 colonies are identified and
>confirmed with biochemical and serological tests.
>Immunomagnetic separation offers a way of rapidly extracting E.coli O157
>from non-target bacteria, resulting in improved rates of isolation. Other
>benefits include a 10-fold concentration of E.coli O157 and easy to
>visualise separation of beads. The Captivate separation is highly
>strain-specific and results in less than 1% carry over of non-O157 E.coli
>from mixed cultures.
>Captivate is available in packs of both 50 and 250 tests.
>- ends ­
>Editor: For more information, please contact: Phil Smith, IDG (UK) Ltd,
>Topley House, 52 Wash Lane, Bury, Lancs, BL9 6AU
>Phone: +44 (0) 161 797 5729, Fax: +44 (0) 161 762 9322
>Email: pasmith at idgplc.com

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