On Wed, 24 Nov 1999 08:48:26 +0100, Per Mygind <perm at biobase.dk>
wrote:
>daniboyton at my-deja.com wrote:
>>> Can anyone please tell me what the melting points for GC and AT pairs in
>> primers are. I believe they are 2 and 4 degrees respectively but I could
>> be wrong. Thanks
>>>> Sent via Deja.com http://www.deja.com/>> Before you buy.
>IMHO 2 degrees for AT and 4 degrees for GC,
try 4 degrees below the calculated value for first experiments.
should be correct.
Yours,
Uli Maier
(7 years PCR practise)
>
