What I always did was autoclave the tubes with the durham tubes in... and
leave them with the air inside. At the end of the autoclave cycle, the air
is sucked out of them.
Daniel Elad wrote in message <38250B86.6BBB772D at agri.huji.ac.il>...
>Try tilting the broth tube as much as you can while inserting or pushing
the
>Durham into it. Even if some gas is trapped, you'll still be able to notice
the
>difference.
>>StMattsson wrote:
>>> I am trying to figure out how Durham tubes are placed in fermentation
tube
>> preparations. (Yes, I know, "upside down". What I want to know is how
they are
>> placed in the broth without any trapped gas.) An answer or direction to a
>> source would be greatly appreciated. Thanks!
>
