If you use a phase contrast microscope, the spores will look very "bright"
compared to the vegetative cells. This method does not require staining.
Rich Mateles
Diego Bonatto - 219 wrote in message <14640337F8 at dna.cbiot.ufrgs.br>...
>Hello people,
>> I'm working with a Bacillus sp. isolated from soil. I'm trying to
>identified the endospore of it using the Schaeffer-Fulton technique
>for light microscopic staining of endospore (green malachite for 10
>minutes and fucsine for 30 seconds). In resume I can not seen any
>endospore in microscopy using this protocol. Is this the best way
>to identify a endospore? I now that are methods more drastics, like
>to submit a culture of Bacillus at 80 degrees for 10 minutes and
>inoculate a culture media with these "cook" endospores. But I want
>a light microscopic staining method (I want to see an endospore
>inside the cell!!). If you know a method for staining endospore
>please send me a reply.
> Another question: are there good references for taxonomy of
>Bacillus? The Bergey's Manual of Determinative Bacteriology is
>very limited and I don't have the The Bergey's books of Systematic
>Bacteriology! Any reference is welcome, specially references that
>deal with fenotypic characters of Bacillus megaterium and Bacillus
>subtilis and methods for identification of these species of Bacillus.
> Thanks
> Diego Bonatto
>diego at dna.cbiot.ufrgs.br>>>Diego Bonatto
>Universidade Federal do Rio Grande do Sul
>Centro de Biotecnologia do Estado do Rio Grande do Sul
>Departamento de Biologia Celular e Molecular
>Brasil
