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A range of organisms should be used for this type of evaluation. As a =
recommendation I would suggest using those stated in the US Pharmacopeia =
for challenge testing ie. S. aureus, E.coli, Ps. aeruginosa, C. albicans =
and Aspergillus. The best media to use would be those recommended for =
antibiotic sensitivity testing eg. Mueller Hinton, Nusens or Sensitivity =
Test Agar, as they do not contain antagonists.
The disc diffusion method may not be the best for this application as =
the oils will not diffuse well. It may be better to use a standard =
challenge test or preservative efficacy test. These involve inoculation =
of a known number of organisms into a solution containing the =
anti-microbial. The mixture is then sampled at intervals and a decrease =
in numbers should be observed.
IDG (UK) can provide both the cultures and the necessary media and in =
addition will offer advice on methodology. IDG (UK) can be contacted on =
Tel: 0044 161 797 5729, email idg at idgplc.com
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<DIV><FONT color=3D#000000 face=3D"Times New Roman">A range of organisms =
should be=20
used for this type of evaluation. As a recommendation I would suggest =
using=20
those stated in the US Pharmacopeia for challenge testing ie. S. aureus, =
E.coli,=20
Ps. aeruginosa, C. albicans and Aspergillus. The best media to use would =
be=20
those recommended for antibiotic sensitivity testing eg. Mueller Hinton, =
Nusens=20
or Sensitivity Test Agar, as they do not contain =
antagonists.</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D"Times New Roman">The disc diffusion =
method may=20
not be the best for this application as the oils will not diffuse well. =
It may=20
be better to use a standard challenge test or preservative efficacy =
test. These=20
involve inoculation of a known number of organisms into a solution =
containing=20
the anti-microbial. The mixture is then sampled at intervals and a =
decrease in=20
numbers should be observed.</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D"Times New Roman">IDG (UK) can provide =
both the=20
cultures and the necessary media and in addition will offer advice on=20
methodology. IDG (UK) can be contacted on Tel: 0044 161 797 5729, email=20
idg at idgplc.com</FONT></DIV></BODY></HTML>
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