hi!
i read a article about PFGE with xanthomonas being digest with the XbaI
enzyme and they got lot of bands.
i dont have the equipement for PFGE so i try to digest DNA from 10 samples
of Xanthomonas with XbaI for 20 hours like the PFGE protocol and made a
normal agarose gel but i didnt get any band. I also try DraI but got the
same results.
It work with other enzymes like BamHI and EcoRI but i got too many bands.
I want to know if its normal that my DNA wasnt digest with the enzyme XbaI ?
Does the PFGE have an effect on the digestion of DNA?
Hope someone can help!!!!
Thanks a lot!
