Hello people,
I'm working with a Bacillus sp. isolated from soil. I'm trying to
identified the endospore of it using the Schaeffer-Fulton technique
for light microscopic staining of endospore (green malachite for 10
minutes and fucsine for 30 seconds). In resume I can not seen any
endospore in microscopy using this protocol. Is this the best way
to identify a endospore? I now that are methods more drastics, like
to submit a culture of Bacillus at 80 degrees for 10 minutes and
inoculate a culture media with these "cook" endospores. But I want
a light microscopic staining method (I want to see an endospore
inside the cell!!). If you know a method for staining endospore
please send me a reply.
Another question: are there good references for taxonomy of
Bacillus? The Bergey's Manual of Determinative Bacteriology is
very limited and I don't have the The Bergey's books of Systematic
Bacteriology! Any reference is welcome, specially references that
deal with fenotypic characters of Bacillus megaterium and Bacillus
subtilis and methods for identification of these species of Bacillus.
Thanks
Diego Bonatto
diego at dna.cbiot.ufrgs.br
Diego Bonatto
Universidade Federal do Rio Grande do Sul
Centro de Biotecnologia do Estado do Rio Grande do Sul
Departamento de Biologia Celular e Molecular
Brasil
