Microbial Molecular Biologist MS level positions (Two) - DuPont Central
Research & Development
Two positions are currently available for microbial molecular
biologists to join an interactive group in DuPont Central Research &
Development (Wilmington, Delaware). Interest in cellular function is a
prerequisite for these appointments. The individual should have
knowledge of microbial genetics, microbial physiology and biochemistry
with an emphasis on biological regulatory mechanisms including feedback
inhibition, covalent protein modification and transcriptional regulatory
mechanisms such as repression and attenuation. It is anticipated that
the successful individual will perform a variety of molecular biological
techniques including recombinant DNA methodology, strain construction,
small molecule extraction/measurement and RNA extraction/measurement.
Gene expression profiling using DNA arrays and gene fusions will be a
focus. He/She will be use various bioinformatics tools for data
aquisition and analysis.
REQUISITE SKILLS/EXPERIENCE:
- Master's degree in biological science or equivalent on-the-job
experience
- Willingness to work with radioisotopes
- Laboratory experience in: Recombinant DNA technology,
microbiological culture techniques, microbial genetics &
biochemistry.
- Computer skills, including word processing, spreadsheets, database,
graphics and the Internet in both MAC and PC environments
- Desire to present research directions/results in a variety of forums
CONTACT:
If interested, please email your resume to Lisa Huang
(Lisa.L.Huang at usa.dupont.com) and Bob LaRossa
(Robert.A.LaRossa at usa.dupont.com).
Lisa Huang
DuPont Experimental Station
P.O. Box 80173
E173/203
Wilmington, DE, 19880-0173.
Bob LaRossa
DuPont Experimental Station
P.O. Box 80173
E173/216
Wilmington, DE, 19880-0173.
Recent Publications from the LaRossa lab:
LaRossa, R. A. (1999) “Nutritional Mutations” in Encyclopedia of
Genetics, S. Brenner and J. Miller, Eds., Academic Press, New York
LaRossa, R. A., editor (1998) Bioluminescent Methods and Protocols.
Humana Press.
Epelbaum, S., LaRossa, R. A., Van Dyk, T. K., Chipman, D. M. (1998)
Branched chain amino acid synthesis in Salmonella typhimurium: a
quantitative analysis. J. Bacteriol. 180: 4056-4067.
Van Dyk, T. K., Ayers, B. L., Morgan, R. W. and LaRossa, R. A. (1998)
Constricted flux through the branche-chain amino acid biosynthetic
enzyme acetolactate synthase triggers elevated expression of genes
reulated by rpoS and internal acidification. J. Bacteriol. 180:
785-792.
Vollmer, A. C., Belkin, S., Smulski, D. R, Van Dyk, T. K. and LaRossa,
R. A. 1997. Detection of DNA damage by use of Escherichia coli carrying
recA'::lux, uvrA'::lux or alkA'::lux reporter plasmids. Applied and
Environ. Microbiol. 63: 2566-2571.
Belkin, S, Smulski, D. R., Dadon, S., Vollmer,A. C., Van Dyk, T. K. and
LaRossa, R. A. 1997. A panel of stress-responsive luminous bacteria for
toxicity detection. Water Research 31: 3009-3016.
Belkin, S., D. R. Smulski, A. C. Vollmer, T. K. Van Dyk and R. A.
LaRossa. 1996. Oxidative stress detection using Escherichia coli
bearing a katG'::lux fusion. Applied and Environ. Microbiol. 62:
2252-2256.
Representative Publications of Lisa Huang:
Foster, P., Huang, L., Santi, D. V. and Stroud, R. “The Structural
Basis for Pseudouridine Formation in tRNA: Pseudouridine Synthase I at
1.5 Å Resolution,” submitted to Science.
Huang L, Ku J, Pookanjanatavip M, Gu X, Wang D, Greene PJ, Santi DV.
"Identification of two Escherichia coli pseudouridine synthases that
show multisite specificity for 23S RNA," Biochemistry, 1998, 37,
15951-7.
Huang L., Pookanjanatavip, M., Gu, X. and Santi, D. V. "A Conserved
Aspartate of tRNA Pseudouridine Synthase is Essential for Activity and a
Probable Nucleophilic Catalyst," Biochemistry, 1998, 37,344-351.
Huang L., Sera, T. and Schultz, P. G. "A Permutational Approach toward
Protein-DNA Recognition," Proceedings of the National Academy of
Sciences, USA, 1994, 91, 3969-3973.
