Counting Borrelia

Jim Fete jfete at worldnet.att.net
Wed Mar 31 09:47:37 EST 1999

Have you tried a Coulter Z2?

Jay and Nancy Mone wrote in message <199903310239.SAA16620 at net.bio.net>...
>I am interested in quantifying Borrelia burgdorferi in preparation for
>quantitative PCR.  The problem is I can't see them on a hemoacytometer,
>even using phase contrast, so I can't count them.  I tried to solidify
>the media (I by it pre-made) using agarose and gelatin to attempt colony
>counts, but can't get the concentrations high enough without
>significantly diluting the media.  Any suggestions?
>Jay Mone'

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