I am interested in quantifying Borrelia burgdorferi in preparation for
quantitative PCR. The problem is I can't see them on a hemoacytometer,
even using phase contrast, so I can't count them. I tried to solidify
the media (I by it pre-made) using agarose and gelatin to attempt colony
counts, but can't get the concentrations high enough without
significantly diluting the media. Any suggestions?
Jay Mone'
