Counting Borrelia

Jay and Nancy Mone jaymone at PAONLINE.COM
Tue Mar 30 21:40:01 EST 1999

I am interested in quantifying Borrelia burgdorferi in preparation for 
quantitative PCR.  The problem is I can't see them on a hemoacytometer, 
even using phase contrast, so I can't count them.  I tried to solidify 
the media (I by it pre-made) using agarose and gelatin to attempt colony 
counts, but can't get the concentrations high enough without 
significantly diluting the media.  Any suggestions?

Jay Mone'  

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