In article <7dm4ao$jm8$1 at nnrp1.dejanews.com>,
aboo9422 at my-dejanews.com wrote:
> I have a question about B.cereus.
>> I have to count and identify colonies of B.cereus in egg-and-brandy liqueur
> (eggs, sugar and 14 - 20 % alcohol). The medium I use is according to Mossel.
> Now I have the following questions:
>> 1. are the colonies of B.cereus only vegetative forms or also comming from
> spores.
B.cereus does form spores and so colonies might contain spores but this
would depend upon the time of incubation and the composition. If uncubation
is for a short while and medium is at optimal condition then i doubt if there
would be any sporulation
> 2. if so, how do I recognize vegetative forms and colonies comming from
spores
you can test this if you have entire colonies with you. Take a colony on an
agar slant and keep and give it horrible conditions to live in for a small
amount of time if all the cells die there were no spores or better still
take a small part of suspected colony and subculture it the next monochrome
stain it spores do shoe up in monochrome staining as colorless bodies inside
the cell. or use
a part of the original culture itself and test.
> 3. is it possible to find any vegetative forms of B.cereus in egg-and-brandy
> liqueur after the eggs had a warm treatment of 30 minutes at 50-60 degree
> Celsius.
> Thanks for your help.
>> Clara
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